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Related Experiment Video

Updated: Jun 20, 2026

Glycopeptide Capture for Cell Surface Proteomics
10:11

Glycopeptide Capture for Cell Surface Proteomics

Published on: May 9, 2014

Specific enrichment methods for glycoproteome research.

Lijuang Zhang1, Haojie Lu, Pengyuan Yang

  • 1Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200433, China.

Analytical and Bioanalytical Chemistry
|September 5, 2009
PubMed
Summary

Glycosylation, a key protein modification, requires efficient enrichment for proteomic analysis. This study reviews methods for capturing glycopeptides and glycoproteins for mass spectrometry.

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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
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Last Updated: Jun 20, 2026

Glycopeptide Capture for Cell Surface Proteomics
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A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

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Area of Science:

  • Biochemistry
  • Proteomics
  • Glycobiology

Background:

  • Glycosylation is a crucial post-translational modification impacting protein function.
  • Proteomic research increasingly targets glycosylation for biological insights.
  • High-throughput analysis of the glycoproteome necessitates efficient enrichment strategies.

Purpose of the Study:

  • To highlight diverse glycan-capturing methods for specific enrichment of glycopeptides/glycoproteins.
  • To provide an overview of techniques applicable to large-scale glycosylation identification.
  • To discuss strategies for advancing high-throughput glycosylation analysis.

Main Methods:

  • Review of various glycan-capturing anchor-based enrichment strategies.
  • Analysis of methods applied to glyco-specific enrichment.
  • Focus on techniques enabling subsequent mass spectrometric analysis.

Main Results:

  • Demonstration of successful application of different enrichment methods.
  • Examples of glycan-capturing anchors facilitating glycopeptide/glycoprotein isolation.
  • Advancements in strategies for large-scale glycosylation identification.

Conclusions:

  • Effective enrichment methods are vital for high-throughput glycoproteome research.
  • Various glycan-capturing strategies have been developed and validated.
  • Continued development of fast and effective enrichment is key for proteomic analysis.