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Relationships between antigen-specific helper and inducer suppressor T cell hybridomas
V K Kuchroo1, J K Steele, R M O'Hara
1Department of Pathology, Harvard Medical School, Boston, MA 02115.
Journal of Immunology (Baltimore, Md. : 1950)
|July 25, 1990
Summary
Inducer suppressor T cells (Ts1) share traits with helper T (Th) cells but produce unique suppressor factors. These factors, distinct from Th cells, specifically inhibit immune responses during induction.
Area of Science:
- Immunology
- Cell Biology
Background:
- Ts1 cells share characteristics with helper/inducer T cells.
- Understanding the relationship between Ts1 and T helper (Th) cells is crucial.
Purpose of the Study:
- To investigate the relationship between Ts1 cells and Th cells.
- To characterize Ts1 hybridomas for antigen receptors, MHC restriction, and lymphokine production.
Main Methods:
- Fusion of Ts1 cells with TCR-negative BW thymoma to create hybridomas.
- Analysis of T cell receptor (TCR) expression, antigen recognition, and lymphokine production.
- Assay of suppressor factors (TsF1) for in vivo and in vitro immune response modulation.
Main Results:
- Established three Ts1 hybridomas expressing CD3-associated TCR-alpha beta.
- Demonstrated antigen-specific recognition restricted by IE molecules.
- Observed production of IL-2 and IL-4 in two hybridomas upon activation.
- Showcased Ts1 hybridomas producing Ag-specific suppressor factors (TsF1) that inhibit immune responses.
Conclusions:
- Ts1 hybridomas exhibit characteristics similar to Th cells.
- Ts1 cells are related to Th cells but distinguished by their ability to produce Ag-binding suppressor factors.
- These suppressor factors specifically inhibit immune responses during the induction phase.