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Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

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Related Experiment Video

Updated: Jun 20, 2026

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Published on: February 16, 2017

Complex and dynamic patterns of Wnt pathway gene expression in the developing chick forebrain.

Robyn Quinlan¹, Manuela Graf, Ivor Mason

¹MRC Centre for Developmental Neurobiology, New Hunt's House, Guy's Hospital Campus, King's College, London SE1 1UL, UK. rquinlan@ich.ucl.ac.uk

Neural Development

|September 8, 2009

Summary

Wnt signaling is crucial for brain development, but its specific roles remain unclear. This study reveals complex Wnt expression patterns and shows Sonic hedgehog signaling represses Wnt4 in the developing thalamus.

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The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

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Creating Avian Forebrain Chimeras to Assess Facial Development

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Published on: February 18, 2021

Area of Science:

Developmental biology
Neuroscience
Molecular biology

Background:

Wnt signaling is essential for vertebrate embryonic brain development.
Specific Wnt functions and pathway activation in the forebrain are not well understood.
Numerous Wnt ligands are expressed in the embryonic forebrain, with complex interactions.

Purpose of the Study:

To create a comprehensive spatiotemporal atlas of Wnt pathway gene expression during chick forebrain regionalization.
To investigate the regulation of Wnt expression by other signaling pathways.
To elucidate the interaction between Wnt and Sonic hedgehog (Shh) signaling in forebrain development.

Main Methods:

Compilation of a spatiotemporal atlas of Wnt pathway gene expression in the chick embryo.
In ovo electroporation techniques to study gene expression.
Analysis of Wnt4 expression regulation by Shh signaling.

Main Results:

Most Wnt pathway genes exhibit dynamic and complex expression patterns during forebrain regionalization.
Observed expression domains challenge existing models of forebrain compartment boundaries.
Sonic hedgehog (Shh) signaling from the zona limitans intrathalamica (ZLI) negatively regulates Wnt4 expression in the thalamus.

Conclusions:

The developing forebrain is influenced by a dynamic interplay of Wnts and Wnt inhibitors.
Demonstrated cross-regulation between Wnt and Shh signaling pathways during forebrain regionalization.
Shh signaling from the ZLI represses Wnt4, revealing a novel interaction between these key developmental pathways.