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A nonradioactive iodide uptake assay for sodium iodide symporter function.

Fanny Waltz¹, Lucie Pillette, Yves Ambroise

¹Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay (iBiTecS), Service de Chimie Bioorganique et de Marquage, Gif-sur-Yvette F-91191, France.

Analytical Biochemistry

|September 8, 2009

Summary

A new spectrophotometric assay simplifies measuring sodium iodide symporter (NIS) function by tracking iodide accumulation. This cost-effective and safe method enables high-throughput screening of NIS modulators.

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Area of Science:

Biochemistry
Cell Biology
Analytical Chemistry

Background:

The sodium iodide symporter (NIS) is crucial for thyroid hormone synthesis.
Standard radioiodide uptake assays for NIS function are costly and pose safety concerns.
A need exists for a more accessible and scalable method to assess NIS activity.

Purpose of the Study:

To develop a simple, safe, and cost-effective spectrophotometric assay for determining iodide accumulation.
To validate this new assay for screening NIS function modulators.
To compare the new assay's results with the traditional radioiodide uptake method.

Main Methods:

Utilized the Sandell-Kolthoff reaction, where iodide catalyzes the reduction of cerium(IV) to cerium(III).
Employed rat thyroid-derived FRTL5 cells to measure iodide accumulation spectrophotometrically.
Adapted the assay for high-throughput screening, enabling the analysis of over 800 samples daily.

Main Results:

The spectrophotometric assay demonstrated high reproducibility with a Z' factor of 0.70.
The assay accurately measured the potency of known NIS inhibitors, yielding IC(50) values comparable to radioiodide assays.
The method proved suitable for robotic adaptation, facilitating high-throughput screening.

Conclusions:

A novel spectrophotometric assay provides a practical alternative to radioiodide uptake for assessing NIS function.
This assay is suitable for high-throughput screening of compounds modulating NIS activity.
The developed method offers a safe, cost-effective, and reliable approach for NIS research and drug discovery.