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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

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Updated: Jun 20, 2026

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
05:44

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Published on: November 9, 2020

Spliceosome structure: piece by piece.

Dustin B Ritchie1, Matthew J Schellenberg, Andrew M MacMillan

  • 1Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.

Biochimica Et Biophysica Acta
|September 8, 2009
PubMed
Summary
This summary is machine-generated.

RNA splicing is vital for gene expression. Structural biology challenges in understanding the spliceosome

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Area of Science:

  • Structural biology
  • Molecular biology
  • Gene regulation

Background:

  • RNA splicing matures protein-coding RNAs in eukaryotes.
  • The spliceosome, the cellular splicing machinery, is large and complex, posing structural challenges.
  • Splice site recognition and active site architecture are poorly understood.

Purpose of the Study:

  • To elucidate the structural details of splice site recognition.
  • To understand the architecture of the spliceosome active site.
  • To gain insights into the regulation and mechanism of RNA splicing.

Main Methods:

  • X-ray crystallography
  • Nuclear Magnetic Resonance (NMR) spectroscopy
  • Biochemical and genetic analyses

Main Results:

  • Defined structures of individual spliceosome domains, isolated proteins, and RNA fragments.
  • Recently determined the structure of the U1 small nuclear ribonucleoprotein (snRNP) complex.
  • Integrated structural data with biochemical and genetic findings.

Conclusions:

  • Structural studies provide critical insights into spliceosome function.
  • Current structural data raise new questions about splicing regulation and mechanism.
  • Further structural investigations are needed to fully understand this essential gene regulatory process.