A maternal high-fat diet is accompanied by alterations in the fetal primate metabolome
James Cox1, Sarah Williams, Kevin Grove
1Metabolomics Core Research Facility, University of Utah Health Sciences, Salt Lake City, UT, USA.
Objective:
To characterize the serum metabolome of a primate model of in utero high-fat exposure.
Study Design:
Serum from maternal and fetal (e130) macaque monkeys exposed to either a high-fat or control diet were analyzed by gas chromatography-mass spectrometry. Multivariate data analysis was performed to reduce the generated data set. Candidate metabolites were further analyzed for significance by using the analysis of variance and comparative t tests.
Results:
Approximately 1300 chromatographic features were detected. Through multivariate data analysis this number was reduced to 60 possible metabolites. With the use of comparative t tests, 22 metabolites had statistical significance (P < .05) over the entire study. By virtue of maternal high-fat diet alone, fetal phenotypic differences are accompanied by altered metabolite concentrations of 7 metabolites (P < .05).
Conclusion:
In utero high-fat diet exposure is associated with an altered fetal epigenome and parlays a characteristic modification in the fetal metabolite profile.
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