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Related Concept Videos

IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
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Notch Signaling Pathway03:14

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
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Propagation of Action Potentials01:23

Propagation of Action Potentials

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Related Experiment Video

Updated: Jun 20, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10:45

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

Recent progress on STIM1 domains controlling Orai activation.

R Schindl1, M Muik, M Fahrner

  • 1Institute of Biophysics, University of Linz, A-4040 Linz, Austria. rainer.schindl@jku.at

Cell Calcium
|September 8, 2009
PubMed
Summary
This summary is machine-generated.

Store-operated calcium entry in non-excitable cells relies on STIM1 and Orai1. Recent research focuses on STIM1

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Related Experiment Videos

Last Updated: Jun 20, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Published on: July 26, 2017

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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
07:17

Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Store-operated calcium (Ca2+) entry is crucial for non-excitable cells.
  • The CRAC channel, comprising STIM1 and Orai1, mediates this process.
  • STIM1 (stromal interaction molecule 1) senses ER Ca2+ depletion and interacts with Orai1 (calcium release-activated calcium channel protein 1) in the plasma membrane to activate Ca2+ influx.

Purpose of the Study:

  • To review recent advancements in understanding the molecular mechanisms of STIM1-Orai1 interaction.
  • To highlight specific domains within the cytosolic part of STIM1 that regulate this crucial coupling.

Main Methods:

  • Literature review of recent studies on STIM1 and Orai1.
  • Analysis of domain interactions within the STIM1 protein.

Main Results:

  • The STIM1-Orai1 interaction is primarily mediated through their C-terminal regions.
  • Specific domains within the cytosolic portion of STIM1 are critical for governing the coupling and activation of Orai1 channels.
  • Recent findings shed light on the structural and functional significance of these STIM1 domains.

Conclusions:

  • Understanding the STIM1-Orai1 interaction domains is key to elucidating store-operated calcium entry.
  • This knowledge may pave the way for therapeutic interventions targeting calcium signaling pathways.