Related Experiment Video
Updated: Jun 20, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Increased low-density lipoprotein oxidation, but not total plasma protein oxidation, in Alzheimer's disease
Sarah Aldred1, Stuart Bennett, Patrizia Mecocci
1School of Sport and Exercise Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. s.aldred.1@bham.ac.uk
Objectives:
The two most common forms of dementia are Alzheimer's disease (AD), and vascular dementia (VaD). In the overlap of biochemical processes which have been identified in AD and VaD, oxidative stress is believed to contribute to the numerous pathologies of both dementias.
Design And Methods:
This study assessed oxidative damage in total plasma proteins, and isolated LDL in AD patients and age matched controls, in addition total antioxidant capacity (TAC) was measured.
Results:
Significantly higher LDL protein carbonylation was observed in AD compared to age-matched controls (AD: 4.17+/-0.73 vs. control: 3.85+/-0.86 nmol/mg LDL; p=0.05, 2-tailed Mann-Whitney), in addition to reduced TAC (AD: 924.708+/-174.429 vs. control: 1078.536+/-252.633 microM; p=0.001, 2-tailed Mann-Whitney). No differences were seen in total plasma protein carbonyl content (AD: 3.88+/-0.31 vs. control: 3.98+/-0.48 nmol/mg protein).
Conclusion:
The results further support the view that oxidation events in AD may be specific in nature, and represent functional changes to proteins, rather than random global events.
Related Concept Videos
Alzheimer Disease ll: Pathophysiology
Alzheimer Disease l: Introduction
Alzheimer's Disease: Overview
The clinical diagnosis of AD hinges on the presence of memory and other cognitive impairments. Biomarkers, such as changes in Aβ and tau...
Alzheimer's Disease: Treatment
Dementia l: Introduction
Atherosclerosis I: Introduction
