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Updated: Jun 20, 2026

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
Published on: March 12, 2019
The effects of prolactin and corticosterone on insulin binding to rat Leydig cells
Muthukumar Karthikeyan1, Jagadeesan Arunakaran, Karundevi Balasubramanian
1Reproductive Medicine Unit, Department of Obstetrics & Gynecology, Christian Medical College & Hospital, Vellore-4,Tamilnadu, India. mmuutthhuu@yahoo.com
Abstract:
The influence of prolactin (PRL) and corticosterone on insulin binding to purified rat Leydig cells was assessed in vitro. The lowest dose of PRL (50 ng/ml) increased (p<0.05) and the remaining PRL concentrations (100, 150, 200, 250 ng/ml) decreased (p<0.05) the insulin binding to Leydig cells. All doses of corticosterone (150, 200, 250, 300 ng/ml) except the lowest one (100 ng/ml) decreased the insulin binding. In conclusion, hyperprolactinemia or excess glucocorticoids associated with an impairment of testicular steroidogenesis may be mediated by a defective insulin binding to Leydig cells.
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