Friend virus replication in normal and immunosuppressed C57BL/6 mice
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H C Van der Gaag1, A A Axelrad
1Department of Anatomy, University of Toronto, Canada.
Abstract:
Young adult C57BL/6 mice are resistant to the replication of Friend virus. We show here that this resistance is not absolute. In 7-week old C57BL/6 mice injected with NB-tropic Friend virus iv, high titers of SFFV could be recovered from the spleen at 8 days after infection but by 21 days, no virus was detectable. A single dose of anti-Thy 1.2 monoclonal antibody iv before FV infection permitted continued replication of SFFV in these animals. This finding strongly supports the hypothesis that SFFV replication in C57BL/6 mice is restricted by a T cell-mediated immune response.
Insights
C57BL/6 mice resistance to Friend virus (FV) is not absolute. T cell depletion allowed sustained spleen infection by spleen-focus forming virus (SFFV), indicating a T cell-mediated immune response restricts FV replication.
Area of Science:
- Immunology
- Virology
- Mouse Models
Background:
- C57BL/6 mice exhibit resistance to Friend virus (FV) replication.
- The mechanisms underlying this resistance are not fully elucidated.
Purpose of the Study:
- To investigate the role of T cells in restricting SFFV replication in C57BL/6 mice.
- To determine if T cell-mediated immunity is the primary mechanism of resistance.
Main Methods:
- Infection of young adult C57BL/6 mice with NB-tropic FV.
- Monitoring of spleen virus titers at 8 and 21 days post-infection.
- Administration of anti-Thy 1.2 monoclonal antibody to deplete T cells prior to infection.
Main Results:
- High titers of spleen-focus forming virus (SFFV) were detected in the spleen at 8 days post-infection.
- SFFV was undetectable by day 21 in untreated mice, indicating viral clearance.
- T cell depletion with anti-Thy 1.2 antibody permitted sustained SFFV replication in the spleen.
Conclusions:
- T cell-mediated immune response is crucial for restricting SFFV replication in C57BL/6 mice.
- This study supports the hypothesis that T cells play a key role in the innate resistance of C57BL/6 mice to FV infection.