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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis.

Gilberto E Hernandez1, Tony S Mondala, Steven R Head

  • 1DNA Array Core Facility, The Scripps Research Institute, La Jolla, California 92037, USA. gilberto@scripps.edu

Biotechniques
|September 10, 2009
PubMed
Summary

This study introduces a novel RNA storage medium that stabilizes samples at room temperature, eliminating the need for cold chain management. This breakthrough simplifies RNA handling and shipment for gene expression analysis.

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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • RNA integrity is crucial for accurate gene expression data.
  • Current methods require cold chain management, posing logistical challenges.
  • Existing RNA stabilizers often necessitate cold storage for optimal protection.

Purpose of the Study:

  • To evaluate a novel anhydrobiosis-based RNA storage medium for room temperature sample stabilization.
  • To assess the impact of room temperature storage on RNA integrity and downstream microarray analysis.
  • To compare the efficacy of the novel medium against traditional cold storage methods.

Main Methods:

  • RNA samples were stored dry at room temperature using the novel medium for varying durations.
  • Samples were compared to frozen controls.
  • Recovered RNA was analyzed directly without further purification.
  • Gene expression profiling was performed using microarray analysis.
  • RNA quality was assessed via spectrophotometry and Bioanalyzer analysis.

Main Results:

  • RNA samples stored at room temperature in the novel medium showed no significant degradation or loss of activity.
  • Downstream applications, including microarray analysis, were not inhibited or interfered with.
  • Gene expression profiles from room temperature-stored samples were comparable to those from frozen controls.
  • Spectrophotometry and Bioanalyzer analysis confirmed RNA quality was identical to controls.

Conclusions:

  • The novel RNA storage medium effectively stabilizes RNA at ambient temperatures, preserving sample integrity.
  • This anhydrobiosis-based approach eliminates the need for cold chain, simplifying sample handling and transport.
  • The technology offers significant advantages for gene expression studies, particularly for remote sample collection and shipment to core facilities.