Related Experiment Video
Updated: Aug 15, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
Published on: July 22, 2011
Salmonellae of serotypes gallinarum and pullorum grouped by biotyping and fimbrial-gene probing
1Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital.
Abstract:
When salmonellae of serotypes Gallinarum (50 isolates) and Pullorum (36 isolates), that produce non-adhesive (type-2) fimbriae, were tested for their reactions in biochemical tests, 81 (94%) were found to belong to three distinct biochemical groups, I-III. Interaction of HinfI-digested DNA of both Gallinarum and Pullorum with a probe of accessory genes of type-1 fimbriation in serotype Typhimurium gave one type of Southern hybridisation pattern that was readily distinguished from that of Typhimurium strains. With a probe of the Typhimurium fimbrial subunit gene, Pullorum isolates were separated into strongly and weakly probe-reactive groups which showed restriction fragment-length polymorphism; these latter groups corresponded to biochemical groups II and III, respectively.
Related Concept Videos
Special Staining Techniques
Fimbriae, Pili, and Axial Filaments
Conjugation
Methods of Classification and Identification
Modern Molecular Taxonomy
Evolution of Microbial Genome

