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Using En Face Immunofluorescence Staining to Observe Vascular Endothelial Cells Directly
Published on: August 20, 2019
[Expression of ICOSL on human coronary artery endothelial cells and its being interferentialed by ox-LDL]
Lin Xu1, Zhi-Liang Li, Xiao-Xing Zhu
1Department of Cardiology, Guangzhou General Hospital of Guangzhou Military Direct, Guangzhou 510010, China.
Aim:
To study the expression of inducible co-stimulator ligand(ICOSL) on human coronary artery endothelial cells(HCAEC) and its being interferentialed by oxidized low density lipoprotein(ox-LDL).
Methods:
ICOSL expression levels were determined by the fluorescence, reverse transcription PCR (RT-PCR) and Western blot, respectively.
Results:
The ICOSL mRNA OD values of control and 100 mg/L ox-LDL group was 0.071+/-0.035 and 0.186+/-0.044, respectively. The Western blot A values of control and 100 mg/L ox-LDL group was 10.88+/-1.53 and 16.03+/-4.08, respectively. ox-LDL increased expression of ICOSL mRNA and protein(P<0.05).
Conclusion:
ICOSL can express on the HCAEC. ox-LDL can up-regulate the expression of ICOSL.
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