Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Whole Body Regeneration01:33

Whole Body Regeneration

Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential; even...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ribosomal protein eL22 contributes to the assembly of 60S ribosomal subunits in <i>Saccharomyces cerevisiae</i>.

bioRxiv : the preprint server for biology·2026
Same author

Ataxin-2-like promotes translation of nonpolyadenylated reovirus mRNA.

Nature communications·2025
Same author

Putting It All Together: The Roles of Ribosomal Proteins in Nucleolar Stages of 60S Ribosomal Assembly in the Yeast <i>Saccharomyces cerevisiae</i>.

Biomolecules·2024
Same author

Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strain.

RNA (New York, N.Y.)·2023
Same author

Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strain.

bioRxiv : the preprint server for biology·2023
Same author

Yeast ribosome biogenesis factors Puf6 and Nog2 and ribosomal proteins uL2 and eL43 act in concert to facilitate the release of nascent large ribosomal subunits from the nucleolus.

Nucleic acids research·2023

Related Experiment Video

Updated: Jun 20, 2026

Spinal Cord Transection In Xenopus laevis Tadpoles
05:54

Spinal Cord Transection In Xenopus laevis Tadpoles

Published on: December 10, 2021

The Rea1 tadpole loses its tail.

Jason Talkish1, John L Woolford

  • 1Department of Biological Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA.

Cell
|September 10, 2009
PubMed
Summary

Researchers identified how yeast ribosome assembly factors are released. The AAA ATPase Rea1 protein uses a mechanochemical process to remove protein complexes from assembling ribosomes, ensuring proper cell function.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Ribosome biogenesis is crucial for protein synthesis and involves over 170 assembly factors in yeast.
  • Assembly factors must be efficiently removed from preribosomes after their roles are completed.
  • Dysregulation of ribosome assembly can lead to cellular dysfunction.

Purpose of the Study:

  • To elucidate the mechanism by which yeast ribosome assembly factors are released from preribosomes.
  • To identify the key protein responsible for the dissociation of assembly complexes.
  • To understand the role of AAA ATPases in ribosome maturation.

Main Methods:

  • Biochemical assays to study protein-protein interactions.
  • In vitro reconstitution of ribosome assembly intermediates.

More Related Videos

Spinal Cord Transection in the Larval Zebrafish
06:57

Spinal Cord Transection in the Larval Zebrafish

Published on: May 21, 2014

Generating Retinal Injury Models in Xenopus Tadpoles
09:29

Generating Retinal Injury Models in Xenopus Tadpoles

Published on: October 13, 2023

Related Experiment Videos

Last Updated: Jun 20, 2026

Spinal Cord Transection In Xenopus laevis Tadpoles
05:54

Spinal Cord Transection In Xenopus laevis Tadpoles

Published on: December 10, 2021

Spinal Cord Transection in the Larval Zebrafish
06:57

Spinal Cord Transection in the Larval Zebrafish

Published on: May 21, 2014

Generating Retinal Injury Models in Xenopus Tadpoles
09:29

Generating Retinal Injury Models in Xenopus Tadpoles

Published on: October 13, 2023

  • Analysis of AAA ATPase activity and its effect on complex stability.

    • Main Results:

    • The AAA ATPase Rea1 was identified as the primary factor mediating the release of assembly protein complexes.
    • Rea1 utilizes a mechanochemical process to actively displace these factors from the preribosome.
    • This release is essential for the timely maturation of functional ribosomes.

    Conclusions:

    • Rea1-mediated dissociation is a critical step in yeast ribosome biogenesis.
    • The AAA ATPase family plays a significant role in protein complex remodeling during ribosome assembly.
    • Understanding this process provides insights into cellular quality control mechanisms.