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N-terminal specific point-immobilization of active proteins by the one-pot NEXT-A method

Keitaro Ebisu1, Hiroaki Tateno, Hiroyuki Kuroiwa

  • 1Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.

Chembiochem : a European Journal of Chemical Biology
|September 10, 2009
PubMed
Abstract

No abstract available in PubMed .

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Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...

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