Structure of a novel highly branched alpha-glucan enzymatically produced from maltodextrin
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Updated: Jun 20, 2026

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method
Published on: March 31, 2022
Keiji Tsusaki1, Hikaru Watanabe, Tomoyuki Nishimoto
1Glycoscience Institute, Hayashibara Biochemical Laboratories, Inc., Naka-ku, Okayama 702-8006, Japan. tsusaki@hayashibara.co.jp
Abstract:
The bacterial strain PP710, isolated from soil and identified as Paenibacillus species, produced a low-digestibility alpha-glucan containing a large amylase-resistant portion. This alpha-glucan was obtained in high yields from maltodextrin (dextrose equivalent 3) by using the condensed culture supernatant of the strain as the enzyme preparation. The water-soluble dietary fiber content of the low-digestibility alpha-glucan was 80.2%, and showed resistance to a rat intestinal enzyme preparation. The alpha-glucan was found to be a novel highly branched alpha-glucan by acid hydrolysis, NMR analysis, gel permeation chromatography, methylation analysis, and enzymatic digestion.
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