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Updated: Jun 20, 2026

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Published on: March 26, 2019
Heats of mixing using an isothermal titration calorimeter: associated thermal effects
Manuel Rodríguez de Rivera1, Fabiola Socorro1, José S Matos1
1Departamento de Física, Universidad de Las Palmas de Gran Canaria, E-35017 Las Palmas de Gran Canaria, Spain.
Abstract:
The correct determination of the energy generated or absorbed in the sample cell of an Isothermal Titration Calorimeter (ITC) requires a thorough analysis of the calorimetric signal. This means the identification and quantification of any thermal effect inherent to the working method. In this work, it is carried out a review on several thermal effects, studied by us in previous work, and which appear when an ITC is used for measuring the heats of mixing of liquids in a continuous mode. These effects are due to: (i) the difference between the temperature of the injected liquid and the temperature of the mixture during the mixing process, (ii) the increase of the liquid volume located in the mixing cell and (iii) the stirring velocity. Besides, methods for the identification and quantification of the mentioned effects are suggested.
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