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Immunological selection of tumour cells which have lost SV40 antigen expression
Nature
|September 1, 1977
Abstract:
In an already tumorigenic spontaneously transformed mouse cell, after further transformation by SV40, the virus-specific antigenic function becomes dominant. By transplantation into syngeneic mice SV40 antigen negative revertant tumour cells can be selected out.
Insights
Further Simian virus 40 (SV40) transformation makes virus-specific antigen dominant in tumorigenic mouse cells. SV40 antigen-negative revertant tumor cells can be selected by transplantation into syngeneic mice.
Area of Science:
- Oncology
- Virology
- Cell Biology
Background:
- Spontaneously transformed mouse cells exhibit tumorigenic properties.
- Simian virus 40 (SV40) is known to induce tumors and alter cellular functions.
Purpose of the Study:
- To investigate the effect of SV40 transformation on already tumorigenic mouse cells.
- To determine if virus-specific antigenic function becomes dominant after SV40 infection.
- To explore methods for selecting SV40 antigen-negative revertant tumor cells.
Main Methods:
- Transformation of pre-existing tumorigenic mouse cells with SV40.
- Assessment of virus-specific antigenic function in transformed cells.
- Transplantation of transformed cells into syngeneic mice to select for revertant phenotypes.
Main Results:
- SV40 transformation led to the dominance of virus-specific antigenic function in the mouse cells.
- Transplantation into syngeneic mice allowed for the selection of SV40 antigen-negative revertant tumor cells.
Conclusions:
- SV40 infection can alter the antigenic profile of tumorigenic cells, emphasizing viral antigens.
- Tumorigenic cells can revert to an antigen-negative state, which can be identified through in vivo selection methods.