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Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Related Experiment Video

Updated: Jun 20, 2026

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
10:54

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Yeast proteome map (last update).

Michel Perrot1, Suzette Moes, Aurélie Massoni

  • 1IBGC, UMR CNRS 5095 Bordeaux, France.

Proteomics
|September 11, 2009
PubMed
Summary

Researchers identified 100 new yeast protein spots using mass spectrometry, expanding the Saccharomyces cerevisiae proteome map to 716 spots and 485 unique proteins, including 363 protein isoforms for deeper proteomic insights.

Area of Science:

  • Proteomics
  • Molecular Biology
  • Yeast Genetics

Background:

  • Two-dimensional gel electrophoresis (2-D PAGE) is a powerful technique for separating complex protein mixtures.
  • Comprehensive protein identification is crucial for advancing proteomic studies.
  • Systematic characterization of the Saccharomyces cerevisiae proteome is ongoing.

Purpose of the Study:

  • To systematically identify previously uncharacterized Saccharomyces cerevisiae proteins separated by 2-D PAGE.
  • To expand the existing yeast proteome map with novel protein identifications.
  • To create a detailed map of yeast protein isoforms.

Main Methods:

  • Mass spectrometry-based protein identification.
  • Two-dimensional gel electrophoresis (2-D PAGE) for protein separation.

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Identification of protein complexes with quantitative proteomics in S. cerevisiae

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Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

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Last Updated: Jun 20, 2026

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
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Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Identification of protein complexes with quantitative proteomics in S. cerevisiae
11:12

Identification of protein complexes with quantitative proteomics in S. cerevisiae

Published on: March 4, 2009

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

  • Bioinformatic analysis for protein and isoform mapping.

    • Main Results:

    • Identification of 100 novel yeast protein spots, many previously uncharacterized due to low abundance.
    • Expansion of the identified yeast 2-D proteome map to 716 protein spots, representing 485 unique proteins.
    • Detailed mapping of 363 protein isoforms, providing a deeper understanding of yeast proteome complexity.

    Conclusions:

    • The study significantly enhances the Saccharomyces cerevisiae proteome map by identifying novel proteins and isoforms.
    • The expanded map provides a valuable resource for yeast research and proteomic investigations.
    • Accessible online resources (Yeast Protein Map server) facilitate further exploration of the yeast proteome.