Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Apolipoprotein J: structure and tissue distribution.

H V de Silva1, J A Harmony, W D Stuart

  • 1Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0575.

Biochemistry
|June 5, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Body fat distribution reference standards in Spanish adolescents: the AVENA Study.

International journal of obesity (2005)·2007
Same author

Anthropometric body fat composition reference values in Spanish adolescents. The AVENA Study.

European journal of clinical nutrition·2005
Same author

Gene therapy and molecular toxicology: a commentary.

Cardiovascular toxicology·2002
Same author

The ADAMs: a new therapeutic avenue?

The pharmacogenomics journal·2002
Same author

[Mycophenolate mofetil in lupus nephritis].

Nefrologia : publicacion oficial de la Sociedad Espanola Nefrologia·2002
Same author

A complement component C3-like protein from the tunicate, Styela plicata.

Developmental and comparative immunology·2002
Same journal

Aromatic Cage-Directed Azide-Methyllysine Photochemistry for Profiling Nonhistone Interacting Partners of the MeCP2 Methyl-CpG-Binding Domain.

Biochemistry·2026
Same journal

Differential Hydroxypyruvate Processing by <i>E. coli</i> and <i>P. aeruginosa</i> DXP Synthases Reveals Preferential Xylulose 5-Phosphate Formation by the <i>P. aeruginosa</i> Enzyme.

Biochemistry·2026
Same journal

Structural and Functional Characterization of Heterologous Nitrogenase Complexes.

Biochemistry·2026
Same journal

Discovery of Bacterial Unspecific Peroxygenases.

Biochemistry·2026
Same journal

Lactate Biology: Subcellular Routing and Chemical Form Define Function.

Biochemistry·2026
Same journal

Nature's Anaerobic Toolkit: Glycyl Radical Enzymes and Their Expanding Functional and Mechanistic Diversity.

Biochemistry·2026
See all related articles

The primary structure of apolipoprotein J (apoJ) was determined, revealing its composition and tissue distribution. This study elucidates apoJ

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Apolipoprotein J (apoJ) is associated with high-density lipoproteins.
  • Its precise structure and function require further elucidation.

Purpose of the Study:

  • To determine the primary structure of apolipoprotein J (apoJ).
  • To investigate the tissue distribution of apoJ mRNA.

Main Methods:

  • Protein sequencing
  • cDNA cloning and sequencing
  • mRNA expression analysis

Main Results:

  • ApoJ is a 427 amino acid polypeptide, cleaved into apoJ alpha and apoJ beta subunits linked by disulfide bonds.
  • Structural analysis predicts amphipathic helices and heparin-binding motifs.

Related Experiment Videos

  • ApoJ mRNA is expressed in various human tissues, with high levels in brain, ovary, testis, and liver.

    • Conclusions:

    • ApoJ possesses a unique structure and tissue distribution compared to other human apolipoproteins.
    • ApoJ is the human homolog of rat sulfated glycoprotein 2.
    • The predicted structural features suggest roles in lipoprotein binding and heparin interaction.