Development of a high-performance liquid chromatography-fluorescence detection method for analyzing
Ake Stenholm1, Sara Holmström, Sandra Hjärthag
1Quality Control Support, GE Healthcare, Björkgatan 30, S-751 84 Uppsala, Sweden. ake.stenholm@ge.com
Abstract:
A novel reversed phase HPLC method for the simultaneous analysis of surfactants containing nonylphenol/dinonylphenol-polyethoxylates and their o-phosphate esters is reported, in which eluting substances are detected fluorescently. Their chemical structures were elucidated by direct infusion electrospray-mass spectrometry in positive mode. The limits of quantification and range of the method were determined to be 0.1mg and 0.1-100 mg surfactant L(-1), respectively, with a reproducibility (RSD) at a concentration of 38 mg surfactant L(-1) of 5.6%. The accuracy was determined by spiking selected process water samples with known amounts of surfactant, and recoveries were typically in the 82-102% range.
