Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence that zymocin-like killer plasmids were present in the common ancestor of terrestrial fungi.

Genome biology and evolution·2026
Same author

The molecular determinants of PABPC-mediated deadenylation rate.

bioRxiv : the preprint server for biology·2026
Same author

Global stabilization of the transcriptome in mitotic cells.

The EMBO journal·2026
Same author

Rapid and repeated evolution of myosin copy number in threespine stickleback.

Current biology : CB·2026
Same author

mRNA 3' UTRs direct microRNA degradation to participate in imprinted gene networks and regulate growth.

Genes & development·2026
Same author

The E3 ubiquitin ligase mechanism specifying targeted microRNA degradation.

Nature·2026
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

RNAi in budding yeast.

Ines A Drinnenberg1,2, David E Weinberg1,2,3, Kathleen T Xie1,2,3

  • 1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|September 12, 2009
PubMed
Summary
This summary is machine-generated.

RNA interference (RNAi) is present in budding yeasts like Saccharomyces castellii, using novel Dicer proteins. Reconstituting RNAi in Saccharomyces cerevisiae silences retrotransposons, offering new research tools.

More Related Videos

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Related Experiment Videos

Last Updated: Jun 20, 2026

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
16:42

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides

Published on: November 24, 2010

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • RNA interference (RNAi) is a conserved gene-silencing mechanism in eukaryotes.
  • The RNAi pathway has been notably absent in the model organism Saccharomyces cerevisiae.
  • Previous research indicated a loss of RNAi in certain budding yeast species.

Purpose of the Study:

  • To investigate the presence and mechanism of RNAi in budding yeast species beyond Saccharomyces cerevisiae.
  • To identify the specific proteins involved in RNAi in these species.
  • To explore the potential of reconstituting RNAi in Saccharomyces cerevisiae for studying gene silencing.

Main Methods:

  • Comparative genomics and molecular analysis of RNAi pathway components in Saccharomyces castellii and Candida albicans.
  • Identification and characterization of noncanonical Dicer proteins.
  • Functional assays to assess RNAi activity and target gene silencing.
  • Genetic manipulation of Saccharomyces cerevisiae to introduce and test RNAi components from S. castellii.

Main Results:

  • RNAi was detected in Saccharomyces castellii and Candida albicans, utilizing noncanonical Dicer proteins.
  • Small interfering RNAs generated in these species primarily target transposable elements and Y' subtelomeric repeats.
  • RNAi-deficient mutants in S. castellii exhibited elevated Y' messenger RNA levels.
  • Introduction of S. castellii Dicer and Argonaute restored RNAi in Saccharomyces cerevisiae, leading to the silencing of endogenous retrotransposons.

Conclusions:

  • A novel class of Dicer proteins functional in RNAi has been identified in budding yeasts.
  • The study reintroduces the RNAi tool for investigating gene silencing in budding yeasts.
  • This research enables the application of budding yeast systems to study RNAi mechanisms.