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Related Experiment Videos

CD27 induction on thymocytes.

J Martorell1, I Rojo, R Vilella

  • 1Servei Immunologia, Hospital Clinic i Provincial de Barcelona, Spain.

Journal of Immunology (Baltimore, Md. : 1950)
|September 1, 1990
PubMed
Summary
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CD27 antigen expression in thymocytes is linked to T cell activation and maturation. Its induction is dependent on T cell receptor signaling and can be modulated by cyclosporin A, suggesting a role in T cell development.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD27 is a T cell surface antigen expressed on mature T cells and a subset of thymocytes.
  • CD27 expression increases upon T cell activation, and CD27- cells can become CD27+ after activation.

Purpose of the Study:

  • To investigate the relationship between CD27 expression and T cell activation and maturation processes.
  • To analyze the induction of CD27 in thymocytes.

Main Methods:

  • Flow cytometry was used to analyze CD27 expression on thymocytes.
  • Thymocytes were stimulated with Concanavalin A (Con A), phorbol 12-myristate 13-acetate (PMA) plus ionomycin, and/or recombinant interleukin-2 (rIL-2).
  • The effect of cyclosporin A on CD27 induction was assessed.

Main Results:

Related Experiment Videos

  • CD27 expression was restricted to thymocytes with high CD3 antigen density.
  • CD27 induction was observed in CD4+CD8+ double-positive thymocytes with low CD3 density upon stimulation with Con A or PMA/ionomycin.
  • PMA with rIL-2 induced CD25 and CD71 but not CD27 expression.
  • Cyclosporin A inhibited CD27 induction by Con A and PMA/ionomycin, while not affecting CD25 expression.

Conclusions:

  • CD27 expression in thymocytes is associated with T cell activation and maturation.
  • The induction of CD27 is dependent on T cell receptor signaling pathways.
  • Cyclosporin A's inhibition of CD27 induction suggests its involvement in T cell maturation processes.