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Updated: Jun 20, 2026
Nuclear Isolation from Cryopreserved In Vitro Derived Blood Cells
Published on: March 15, 2024
Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage
S R Burger1, A Hubel, J McCullough
1Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Background:
Various tissue culture medium formulations currently are used to transport, store and process stem and progenitor cells for transplantation, although these solutions are not manufactured for clinical use.
Methods:
We have developed an infusible-grade solution, STM-Sav, for short-term liquid cell storage and tested its ability to maintain viable functional hematopoietic cells, compared with commonly-used tissue culture solutions. G-CSF-stimulated normal-donor PBPC were stored in alpha-MEM, IMDM, RPMI-1640, AIM 5, X-VIVO 10, PlasmaLyte A and STM-Sav, in gas-permeable Cryocyte bags, at ambient temperature and 4 degrees C. The percentage of viable cells, percent recovery of viable mononuclear cells (MNC), percentage of CD34+ cells, CFU-GM frequency and solution pH were determined for each solution, at time points ranging from 0 to 72 h.
Results:
Cells were slightly better maintained at 4 degrees C than at ambient temperature. No solution was superior to any other overall, although PlasmaLyte A was significantly inferior to all other solutions at preserving viable MNC. STM-Sav consistently maintained viable, functional cells as well as, or better than in-vitro-use-only culture media, or PlasmaLyte A.
Discussion:
Cells could be stored in STM-Sav for at least 24 h at 4 degrees C or ambient temperature, with recovery of approximately 90% of initial viable MNC, unchanged percentage CD34+ cells and stable solution pH.
