Sensitivity of fluorophore-quencher labeled microbubbles to externally applied static pressure

A fluorophore-quencher (F-Q) labeled microbubble system is proposed as a sensor for measuring externally applied static pressure distribution in a tumor. To quantify the sensitivity of such an F-Q bubble system to the externally applied pressure, a model describing bubble response to the static pressure was derived. Additionally, a model connecting the fluorescence lifetime and bubble radius was developed for the basic F-Q bubble system. The sensitivity is quantified based on these models given typical parameters. Results show that it is possible to resolve as low as 1 mm Hg pressure variation when both the F-Q bubble system and the measurement system are optimized. Strategies for optimizing an F-Q bubble system are discussed.