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A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
Published on: December 27, 2013
Synthetic substrate for application in both high and low throughput assays for botulinum neurotoxin B protease
Nicholas T Salzameda1, Joseph T Barbieri, Kim D Janda
1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
Bioorganic & Medicinal Chemistry Letters
|September 15, 2009
Abstract:
A FRET peptide substrate was synthesized and evaluated for enzymatic cleavage by the BoNT/B light chain protease. The FRET substrate was found to be useful in both a high throughput assay to uncover initial 'hits' and a low throughput HPLC assay to determine kinetic parameters and modes of inhibition.
