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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
Comparison between pp65 antigenemia assay and quantitative real-time polymerase chain reaction for detection of
Raimond Lugert1, Utz Reichard, Frank T Hufert
1Institute for Medical Microbiology, University Medical Center Göttingen, 37075 Göttingen, Germany. rlugert@gwdg.de
Abstract:
Real-time polymerase chain reaction (PCR) and pp65 antigenemia assay for the detection of active cytomegalovirus infection in immunocompromised patients experiencing neutropenia after bone marrow or kidney transplantation have been compared with a special focus on evaluability and embedment in daily routine diagnostics. Investigating 334 specimens from 97 patients, real-time PCR was shown to be the superior assay with regard to the parameters focused on.
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