Related Experiment Video
Updated: Jun 20, 2026
Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
Enzymatic stereospecific preparation of fluorescent S-adenosyl-L-methionine analogs
Otmar M Ottink1, Frank H T Nelissen, Yvonne Derks
1Institute for Molecules and Materials, Department of Biophysical Chemistry, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands.
Abstract:
S-Adenosyl-L-methionine (SAM) is the preferred cofactor for biological methyl group transfers to various substrates such as nucleic acids, proteins, and lipids. Here we present stereospecific (>95% of the desired enantiomer) and high-yield preparation of four fluorescent and biologically active SAM analogs and demonstrate their usefulness in binding studies. Using a fluorescence titration experiment, we obtained a K(d) of 0.38 microM for the S-2,6-diaminopurinylmethionine-SAM-III riboswitch complex.
