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Glutathione-dependent and ketone body-related enzymes in the selenium-deficient rat
1Department of Genetic and Cellular Toxicology, Wallenberg Laboratory, University of Stockholm, Sweden.
Abstract:
Male rats, born of selenium (Se)-depleted dams and continuously fed a Se-deficient diet regimen or Se-repleted for 11 weeks, were used for some enzymatic determinations in samples from liver, heart, kidney and hind limb muscle. The Se-dependent GSH-Px activity in liver and kidney of the Se-deficient group was less than 0.5% of Se-repleted controls, whereas a 3-fold increase in liver and kidney GSH S-transferase activity was found in these Se-deficient rats. The Se-deficient group excreted 3 times more acetoacetate via the urine than their Se-repleted siblings. The activity of the key enzymes of ketone body utilization was similar in tissues from Se-repleted and Se-deficient rats. It is suggested that longlasting or even Se-unresponsive changes in enzymes of ketone body metabolism might have developed in these rats born of Se-depleted dams.
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