Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Skin sampling-validation of a pad method and comparison with commonly used methods.

A Hambraeus1, J Hoborn, W Whyte

  • 1Department of Clinical Bacteriology, University Hospital, Uppsala, Sweden.

The Journal of Hospital Infection
|July 1, 1990
PubMed
Summary

Two novel skin bacteria sampling pads demonstrated high efficiency, especially when moist. These pads outperformed traditional methods like the bud swab and Rodac plate for bacterial recovery.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proposal for testing ventilation systems to control microbial contamination in surgical operating rooms.

The Journal of hospital infection·2025
Same author

Source strength as a measurement to define the ability of clean air suits to reduce airborne contamination in operating rooms.

The Journal of hospital infection·2021
Same author

An actuatable soft reservoir modulates host foreign body response.

Science robotics·2020
Same author

Criticisms of Teo et al.'s study of ultraclean air systems in operating theatres.

The Journal of hospital infection·2020
Same author

Reply to E. Houltz.

The Journal of hospital infection·2019
Same author

Ultraclean air systems and the claim that laminar airflow systems fail to prevent deep infections after total joint arthroplasty.

The Journal of hospital infection·2019

Area of Science:

  • Microbiology
  • Biomedical Engineering
  • Infection Control

Background:

  • Accurate skin bacteria sampling is crucial for infection control and diagnostics.
  • Traditional sampling methods like bud swabs and Rodac plates have limitations in efficiency and recovery.
  • Developing improved sampling techniques is essential for reliable microbiological assessments.

Purpose of the Study:

  • To evaluate the efficiency of two novel pad types for skin bacteria sampling.
  • To compare the performance of these pads against established methods such as bud swabs, Rodac plates, and scrub cylinders.
  • To determine the optimal conditions for pad-based bacterial sampling.

Main Methods:

  • Two pad types were tested: one viscose-polyester-polyamide blend and one polyvinyl-alcohol foam.

Related Experiment Videos

  • Bacterial sampling efficiency was assessed using both double-sample and consecutive-sample methodologies.
  • Pad efficiency was compared against bud swab, Rodac plate, and scrub cylinder methods.

    • Main Results:

    • Both pad types exhibited equal and high efficiency, particularly when used in a moist state.
    • Using the double-sample method, pads and the scrub cylinder method showed similar efficiency (~50%).
    • Consecutive-sample analysis revealed pads at ~45% efficiency, significantly outperforming the cylinder scrub (29%), bud swab (16%), and Rodac plate (5%).

    Conclusions:

    • The tested pads are highly effective for skin bacteria sampling, offering an improvement over traditional methods.
    • Moistened pads enhance bacterial recovery, suggesting a practical application for clinical settings.
    • The Rodac plate's low efficiency may stem from sampling microcolonies rather than individual bacterial cells.