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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Related Experiment Video

Updated: Jun 20, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

Site-specific modification of RNA by functionality-transfer ODN probes.

Kazumitsu Onizuka1, Yosuke Taniguchi, Takamasa Nishioka

  • 1Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.

Nucleic Acids Symposium Series (2004)
|September 15, 2009
PubMed
Summary

Researchers developed a new method for site-specific RNA modification using oligonucleotide (ODN) probes. This technique enables precise cytosine modifications within RNA molecules, offering potential for novel biological tools and therapies.

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RNA-Associated Chromatin DNA-DNA Interaction Method
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RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

Related Experiment Videos

Last Updated: Jun 20, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
11:37

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Background:

  • Efficient RNA modification is crucial for developing advanced biological tools and therapeutic strategies.
  • Current methods for site-specific RNA modification often face limitations in efficiency and selectivity.

Purpose of the Study:

  • To develop a general and efficient method for site-specific RNA modification.
  • To investigate the use of functionality-transfer oligonucleotide (ODN) probes for precise RNA editing.

Main Methods:

  • Utilized functionality-transfer ODN probes to achieve site-specific RNA modifications.
  • Investigated the mechanism underlying the selectivity of the modification reaction within DNA-RNA duplexes.

Main Results:

  • Successfully achieved site-specific and cytosine-selective RNA modifications.
  • Demonstrated that the selectivity of the reaction is dependent on the close proximity of reactants within DNA-RNA duplexes.

Conclusions:

  • The developed functionality-transfer ODN probe method provides an efficient approach for site-specific RNA modification.
  • This technique offers a promising platform for creating novel RNA-based therapeutics and research tools.