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Updated: Jun 20, 2026

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
Published on: January 11, 2012
DNA ligation using photoremovable protecting groups
Yuta Kawano1, Tadao Takada, Mitsunobu Nakamura
1Graduate School of Engineering, University of Hyogo, Himeji, Hyogo 671-2280, Japan.
Abstract:
Template-directed ligation of oligonucleotides by photochemical reaction has attracted much interest because of its biomedical and synthetic applications. In this study, we developed photoligaton of DNA by using a photoremovable protecting group and thiol-disulfide exchange reaction. A phosphoroamidite of o-nitrobenzyl derivatives were synthesized, and DNA modified with a nitrobenzyl-protected thiol group and disulfide group was synthesized by conventional phosphoroamidite chemistry using a DNA synthesizer. It was shown that photochemical reaction of a nitrobenzyl group with UV irradiation produced a free thiol group, leading to the DNA ligation through the thiol-disulfide exchange reaction.
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