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Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
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Next-generation Sequencing03:00

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Although all next-generation methods use different technologies, they all share a set of standard features.

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Related Experiment Video

Updated: Jun 20, 2026

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
08:56

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

Published on: January 11, 2012

DNA ligation using photoremovable protecting groups.

Yuta Kawano1, Tadao Takada, Mitsunobu Nakamura

  • 1Graduate School of Engineering, University of Hyogo, Himeji, Hyogo 671-2280, Japan.

Nucleic Acids Symposium Series (2004)
|September 15, 2009
PubMed
Summary

This study introduces a novel photochemical method for DNA ligation using a photoremovable protecting group and thiol-disulfide exchange. This technique enables efficient DNA synthesis for various applications.

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Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
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Last Updated: Jun 20, 2026

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
08:56

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

Published on: January 11, 2012

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

Area of Science:

  • Biochemistry
  • Synthetic Chemistry
  • Molecular Biology

Background:

  • Template-directed oligonucleotide ligation is crucial for biomedical and synthetic applications.
  • Photochemical methods offer precise control over chemical reactions.
  • Developing efficient and controlled DNA ligation techniques is an ongoing challenge.

Purpose of the Study:

  • To develop a novel photochemical method for DNA ligation.
  • To utilize a photoremovable protecting group and thiol-disulfide exchange reaction for DNA synthesis.
  • To enable precise and efficient DNA assembly for potential applications.

Main Methods:

  • Synthesis of a phosphoroamidite of o-nitrobenzyl derivatives.
  • DNA modification with a nitrobenzyl-protected thiol group and disulfide group using phosphoroamidite chemistry.
  • Photochemical activation using UV irradiation to generate free thiol groups.

Main Results:

  • Successful synthesis of modified DNA strands.
  • Photochemical reaction efficiently produced free thiol groups upon UV irradiation.
  • Demonstrated DNA ligation through thiol-disulfide exchange reaction.

Conclusions:

  • The developed photochemical method enables efficient DNA ligation.
  • This technique offers a controlled approach for DNA synthesis.
  • The method has potential for diverse biomedical and synthetic applications.