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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 20, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Photo-controllable aptamer.

Shinzi Ogasawara1, Mizuo Maeda

  • 1RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan. o_shinji@riken.jp

Nucleic Acids Symposium Series (2004)
|September 15, 2009
PubMed
Summary

Researchers created a light-controlled method to regulate G-quadruplex structures. This breakthrough allows for reversible switching between stable and unstructured states, impacting aptamer binding.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Photochemistry

Background:

  • G-quadruplexes are crucial nucleic acid structures involved in various biological processes.
  • Controlling G-quadruplex formation and stability is essential for understanding their function and for therapeutic applications.
  • Existing methods for G-quadruplex manipulation often lack precise temporal or spatial control.

Purpose of the Study:

  • To develop a novel method for the light-induced regulation of G-quadruplex formation.
  • To create photo-controllable G-quadruplexes that can reversibly switch between stable and unstructured states.
  • To demonstrate the utility of these photo-controllable G-quadruplexes in regulating aptamer-protein interactions.

Main Methods:

  • Synthesis of a photochromic nucleobase, (8FV)G, capable of cis-trans photoisomerization.

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  • Incorporation of (8FV)G into G-rich sequences to form photo-controllable G-quadruplexes.
  • Characterization of G-quadruplex formation and stability using spectroscopic techniques (e.g., UV-Vis, fluorescence).
  • Demonstration of reversible G-quadruplex aptamer-thrombin binding control using light.

    • Main Results:

    • Successful development of a G-quadruplex system regulated by light via (8FV)G photoisomerization.
    • Achieved straightforward and reversible switching between a stable quadruplex state and a non-structured state.
    • Demonstrated light-dependent, reversible control over the binding of a G-quadruplex aptamer to its target, thrombin.

    Conclusions:

    • The developed method provides a powerful tool for precise, light-based spatiotemporal control over G-quadruplex formation.
    • Photo-controllable G-quadruplexes offer new possibilities for molecular switches, diagnostics, and therapeutics.
    • This work opens avenues for light-activated aptamer-based applications, such as targeted drug delivery or biosensing.