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Updated: Jun 20, 2026

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
Protein kinase Calpha puts the hand cuffs on epidermal keratinocyte proliferation
1Charlie Norwood VA Medical Center, Augusta, Georgia, USA. wbollag@mcg.edu
Abstract:
As the predominant cellular receptor for phorbol esters, protein kinase C (PKC) is assumed to play a role in epidermal carcinogenesis. Nevertheless, determining its exact role in keratinocytes has been difficult because of the existence of multiple PKC isoforms and the inherent weaknesses in methodologies used to investigate their function. In this issue, Jerome-Morais et al. describe their use of multiple in vitro, in situ, overexpression, and knockdown approaches to demonstrate that PKCalpha induces keratinocyte growth arrest.
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