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Related Experiment Video

Updated: Jun 20, 2026

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

Increase in sphingolipid catabolic enzyme activity during aging.

Santosh J Sacket¹, Hae-Young Chung, Fumikazu Okajima

¹Laboratories of Pharmacology, College of Pharmacy (BK21 Project) and Longevity Institute of Life Science and Technology, Pusan National University, Busan 609-735, Republic of Korea.

Acta Pharmacologica Sinica

|September 15, 2009

Summary

Sphingolipid metabolism, involving enzymes like sphingomyelinase and ceramidase, significantly increases during development and aging. This suggests sphingolipids, such as ceramide and sphingosine, play dynamic roles in cellular processes.

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Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

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Published on: April 27, 2021

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Last Updated: Jun 20, 2026

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Preparation of Human Tissues Embedded in Optimal Cutting Temperature Compound for Mass Spectrometry Analysis

Published on: April 27, 2021

Area of Science:

Biochemistry
Cell Biology
Gerontology

Background:

Sphingolipids are crucial cellular components involved in various physiological processes.
Understanding their metabolic regulation is key to comprehending development and aging.

Purpose of the Study:

To investigate the role of sphingolipid metabolism in rat development and aging.
To analyze changes in sphingolipid metabolic enzyme activities across different tissues and age points.

Main Methods:

Systemic analysis of sphingolipid metabolic enzyme activities in rat kidney, liver, and brain tissues.
Tissues were collected from rats aged 1 day to 720 days.

Main Results:

Catabolic enzyme activities (sphingomyelinase, ceramidase) exceeded anabolic activities, suggesting ceramide and sphingosine accumulation.
Enzyme activities significantly increased with age in all tissues, with further elevation by day 720.
Liver showed high neutral-SMase activity; kidney and brain exhibited high neutral-SMase and ceramidase activities.

Conclusions:

Sphingolipid metabolism is dynamically altered during development and aging.
Accumulation of ceramide and sphingosine is indicated by enzyme activity changes.
Sphingolipids likely play critical roles in cell proliferation, differentiation, and apoptosis throughout the lifespan.