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Updated: Jun 20, 2026

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

Is bacteriologic surveillance in endoscope reprocessing stringent enough?

J Kovaleva¹, N E L Meessen, F T M Peters

¹Department of Medical Microbiology, University Medical Centre Groningen, University of Groningen, 9700 RB Groningen, The Netherlands. j.kovaleva@mmb.umcg.nl

|September 15, 2009

Summary

This summary is machine-generated.

A contaminated duodenoscope caused multidrug-resistant Pseudomonas aeruginosa sepsis in three patients. Routine endoscope culturing identified the scope as the infection source, preventing further spread.

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Area of Science:

Infectious Diseases
Medical Device Contamination
Microbiology

Background:

Endoscopes, particularly duodenoscopes, are linked to nosocomial infections.
Endoscopic retrograde cholangiopancreaticography (ERCP) procedures carry a risk of infection transmission.

Observation:

An outbreak of multidrug-resistant Pseudomonas aeruginosa sepsis occurred in three patients post-ERCP.
Molecular typing identified a single ERCP scope as the source of P. aeruginosa transmission.

Findings:

No contamination was found in washer-disinfectors, tubing, or environmental surfaces.
Pseudomonas isolates from patient blood and the endoscope channels before ethylene oxide (ETO) sterilization were molecularly linked.
Routine microbiological surveillance of endoscopes successfully detected the contaminated device.

Implications:

Despite not preventing initial infections, endoscope culturing aids in identifying contamination sources.
This surveillance protocol likely prevented additional cross-contaminations in subsequent ERCP procedures.
Enhanced disinfection and surveillance protocols are crucial for medical device safety.