Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

iChip

iChip

The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Photophysics of Iodine-Substituted Oxyluciferins: Observation of Simultaneous High Fluorescence Quantum Yields and Singlet Oxygen Sensitization.

Journal of the American Chemical Society·2026

Same author

Correction to "Investigation of 6PPD-Quinone in Rubberized Asphalt Concrete Mixtures".

ACS environmental Au·2024

Same author

Reactive Oxygen Species and Chromophoric Dissolved Organic Matter Drive the Aquatic Photochemical Pathways and Photoproducts of 6PPD-quinone under Simulated High-Latitude Conditions.

Environmental science & technology·2023

Same author

Concentrations of Tire Additive Chemicals and Tire Road Wear Particles in an Australian Urban Tributary.

Environmental science & technology·2022

Same author

Syrbactin-class dual constitutive- and immuno-proteasome inhibitor TIR-199 impedes myeloma-mediated bone degeneration in vivo.

Bioscience reports·2022

Same author

Pentadienylnitrobenzyl and Pentadienylnitropiperonyl Photochemically Removable Protecting Groups.

The Journal of organic chemistry·2021

Search research articles

Related Experiment Video

Updated: Jun 20, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

How to make a DNA chip.

Michael C Pirrung¹

¹Department of Chemistry, Levine Science Research Center, Box 90317, Duke University, Durham, NC 27708-0317, USA. pirrung@chem.duke.edu

Angewandte Chemie (International Ed. in English)

|September 16, 2009

Summary

DNA microarrays enable simultaneous analysis of gene expression, advancing biological research. Their versatility is expanding into genotyping and medical diagnostics, with ongoing improvements in production methods.

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Microarrays, often called "DNA chips", are crucial tools in modern biological research.
Initially used for gene expression analysis, their application has broadened significantly.

Purpose of the Study:

To highlight the capabilities of DNA microarrays in gene expression analysis.
To discuss the expanding applications of microarrays beyond nucleic acid analysis.
To emphasize the role of interdisciplinary technologies in advancing microarray production.

Main Methods:

Utilizing photolithographic methods for early microarray preparation.
Leveraging the vast parallelism offered by microarrays for simultaneous gene analysis.
Applying physical sciences and engineering, including chemistry, to improve production.

More Related Videos

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

Published on: March 23, 2012

Related Experiment Videos

Last Updated: Jun 20, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

Published on: March 23, 2012

Main Results:

DNA microarrays facilitate the assessment of gene expression levels.
Microarrays enable simultaneous analysis of numerous genes, aiding in gene function determination.
Applications now include research genotyping and medical genetic diagnosis.

Conclusions:

DNA microarrays demonstrate the power of high-throughput biological analysis.
The development of microarrays is driving innovation in genomics and diagnostics.
Interdisciplinary approaches are essential for future advancements in microarray technology.