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Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
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Lactic acid bacteria population in children diagnosed with human immunodeficiency virus

Leon M T Dicks1, Taryn Fraser, Kim ten Doeschate

  • 1Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa. lmtd@sun.ac.za

Journal of Paediatrics and Child Health
|September 16, 2009
PubMed

Insights

Trimethoprim/sulphamethoxazole treatment significantly reduced intestinal lactic acid bacteria in children. Some resistant strains identified may serve as probiotics to restore gut microbiota balance.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Pharmacology

Background:

  • The intestinal microbiota plays a crucial role in maintaining gut health.
  • Antibiotic use can disrupt the natural balance of gut bacteria.
  • Lactic acid bacteria are beneficial components of the intestinal flora.

Purpose of the Study:

  • To assess the impact of trimethoprim/sulphamethoxazole on intestinal lactic acid bacteria populations.
  • To investigate antibiotic resistance development in these bacteria.

Main Methods:

  • Isolation and identification of lactic acid bacteria from stool samples of 100 children.
  • Biochemical characterization and DNA profiling using PCR.
  • Antibiotic susceptibility testing via the paper-disk method.

Main Results:

  • Common lactic acid bacteria identified included Lactobacillus and Enterococcus species.
  • Significant resistance was observed against sulphamethoxazole (66%), compound sulphonamides (50%), and vancomycin (35%).
  • Most isolates were sensitive to chloramphenicol and streptomycin.

Conclusions:

  • Trimethoprim/sulphamethoxazole treatment markedly decreased intestinal lactic acid bacteria.
  • The emergence of sulphamethoxazole-resistant strains suggests potential for probiotic use to correct dysbiosis.
Abstract

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