Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness and Safety of First-Line Empirical Eradication Therapy with Rebamipide: Results from the European Registry on <i>Helicobacter pylori</i> Management (Hp-EuReg).

Antibiotics (Basel, Switzerland)·2026
Same author

Review Article: Implementation Challenges in Helicobacter pylori Management: Bridging Evidence and Real-World Practice.

Alimentary pharmacology & therapeutics·2026
Same author

Higher Doses of Bismuth and Antibiotics Do Not Improve Helicobacter pylori Treatment Effectiveness: Results From the European Registry on Helicobacter pylori Management.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association·2026
Same author

European Registry on <i>Helicobacter pylori</i> Management (Hp-EuReg): Most relevant results for clinical practice.

Frontiers in gastroenterology (Lausanne, Switzerland)·2026
Same author

<i>Helicobacter pylori</i> Screen-and-Treat Programs for Gastric Cancer Prevention - IARC Working Group Report.

The New England journal of medicine·2026
Same author

Lack of follow-up in <i>Helicobacter pylori</i> eradication treatment: Results from the European registry on <i>Helicobacter pylori</i> management.

World journal of gastroenterology·2026
Same journal

Factors associated with drug-resistant tuberculosis among patients at a tertiary referral hospital in Tanzania.

BMC microbiology·2026
Same journal

Synthesis of zinc oxide nanoparticles and their effect on biofilm of methicillin-resistant Staphylococcus aureus isolates.

BMC microbiology·2026
Same journal

A population-based retrospective machine learning study of COVID-19 severity using integrated clinical and viral genomic data in Jiangsu Province, China.

BMC microbiology·2026
Same journal

Bacillus-based probiotic supplementation reshapes rumen bacterial and fungal communities and enhances carbohydrate-degrading functional capacity in weaned yaks.

BMC microbiology·2026
Same journal

Genome-to-function integrated exploration of polyethylene biodegradation by Rhodococcus opacus R7.

BMC microbiology·2026
Same journal

Overcoming the bottleneck in Treponema pallidum quantification: a novel flow cytometric assay for rapid, precise, and cost-effective detection.

BMC microbiology·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

First multi-locus sequence typing scheme for Arcobacter spp.

William G Miller1, Irene V Wesley, Stephen L W On

  • 1Produce Safety and Microbiology Research Unit, Agricultural Research Service, US Department of Agriculture, Albany, CA 94710, USA. William.Miller@ars.usda.gov

BMC Microbiology
|September 16, 2009
PubMed
Summary
This summary is machine-generated.

A new multilocus sequence typing (MLST) method was developed for Arcobacter species commonly found in food and water. This method effectively differentiates Arcobacter strains, aiding in the study of gastroenteritis outbreaks.

More Related Videos

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Related Experiment Videos

Last Updated: Jun 20, 2026

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Area of Science:

  • Microbiology
  • Genetics
  • Food Safety

Background:

  • Arcobacter species are frequent contaminants in food and water sources.
  • Increasing isolation of Arcobacter species from human diarrheal samples highlights their clinical relevance.

Purpose of the Study:

  • To develop and validate the first multilocus sequence typing (MLST) method for key Arcobacter species.
  • To establish a foundation for epidemiological studies of Arcobacter infections.

Main Methods:

  • A seven-locus MLST scheme, utilizing genes previously employed for Campylobacter species, was adapted for Arcobacter.
  • The method was applied to a diverse collection of 374 Arcobacter isolates, including A. butzleri, A. cryaerophilus, A. skirrowii, A. cibarius, and the novel species A. thereius.

Main Results:

  • The developed MLST method identified a high number of alleles across the seven loci.
  • The majority of the 374 Arcobacter isolates exhibited unique sequence types, indicating excellent discriminatory power.
  • A specific gene, glyA1, was incorporated into the typing method, while glyA2 was excluded due to limited added discrimination.

Conclusions:

  • The Arcobacter MLST method demonstrates significant potential for strain discrimination and epidemiological investigations.
  • No correlation was found between MLST profiles and host or geographical origins in this study.
  • An online Arcobacter MLST database was established to facilitate data sharing and future research.