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Characterization of Aquatic Biofilms with Flow Cytometry
Published on: June 6, 2018
Flow cytometric analysis of benthic prokaryotes attached to sediment particles
Stefano Amalfitano1, Stefano Fazi Alberto Puddu
1Water Research Institute (IRSA-CNR), Via Salaria Km 29.300, Monterotondo, Rome, Italy. amalfitano@irsa.cnr.it
Journal of Microbiological Methods
|September 16, 2009
Abstract:
Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity.
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