Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blood-based RNA-Seq of 5412 individuals with rare disease identifies new candidate diagnoses in the National Genomic Research Library.

medRxiv : the preprint server for health sciences·2026
Same author

SAVANA: reliable analysis of somatic structural variants and copy number aberrations using long-read sequencing.

Nature methods·2025
Same author

Clinical utility of 'Shaken' biopsies for whole-genome sequencing.

Journal of clinical pathology·2025
Same author

Ongoing chromothripsis underpins osteosarcoma genome complexity and clonal evolution.

Cell·2025
Same author

Large-scale analysis of whole genome sequencing data from formalin-fixed paraffin-embedded cancer specimens demonstrates preservation of clinical utility.

Nature communications·2024
Same author

A Comparison of Structural Variant Calling from Short-Read and Nanopore-Based Whole-Genome Sequencing Using Optical Genome Mapping as a Benchmark.

Genes·2024

Related Experiment Video

Updated: Jun 20, 2026

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Pan-vertebrate conserved non-coding sequences associated with developmental regulation.

Greg Elgar1

  • 1School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Rd, London E1 4NS, UK. g.Elgar@qmul.ac.uk

Briefings in Functional Genomics & Proteomics
|September 16, 2009
PubMed
Summary
This summary is machine-generated.

Highly conserved non-coding sequences in vertebrates are challenging to identify but crucial for understanding gene regulation and evolution. Research is unraveling their properties, identification methods, and roles in disease.

More Related Videos

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
07:23

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Published on: July 16, 2010

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
10:23

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

Related Experiment Videos

Last Updated: Jun 20, 2026

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
10:06

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
07:23

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Published on: July 16, 2010

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
10:23

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

Area of Science:

  • Genomics
  • Evolutionary Biology
  • Molecular Biology

Background:

  • Identifying coding sequences in vertebrate genomes is straightforward using computational methods.
  • Non-coding regulatory sequences are difficult to identify due to small size and low sequence homology.
  • A small subset of non-coding sequences exhibits high conservation across vertebrates.

Purpose of the Study:

  • To review the properties of highly conserved non-coding sequences.
  • To discuss methods for their identification and functional assays.
  • To explore their evolution, function, and role in disease.

Main Methods:

  • Review of existing literature on conserved non-coding sequences.
  • Analysis of computational and experimental approaches for identification.
  • Functional assays and evolutionary studies.

Main Results:

  • Highly conserved non-coding sequences possess unique properties enabling their identification.
  • Functional assays reveal their regulatory roles.
  • These elements show evolutionary patterns and are implicated in diseases.

Conclusions:

  • Conserved non-coding regions are vital for molecular-level gene regulation.
  • Understanding their 'language and grammar' is key to deciphering genome function.
  • Further research promises insights into their roles in health and disease.