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Chicken growth hormone: cDNA-synthesis and base sequence.

D Baum1, G Graser, M Heib

  • 1Abteilung Biochemie, Universität Bonn, FRG.

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
|January 1, 1990
PubMed
Summary

Researchers synthesized chicken growth hormone (GH) cDNA, revealing high similarity to previous chicken sequences but notable differences in the non-coding region compared to duck GH. Chicken GH amino acid sequences were identical.

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Area of Science:

  • Molecular Biology
  • Endocrinology
  • Comparative Genomics

Background:

  • Growth hormone (GH) plays a crucial role in animal growth and metabolism.
  • Understanding GH gene evolution provides insights into species-specific adaptations.

Purpose of the Study:

  • To synthesize and analyze chicken growth hormone cDNA.
  • To compare chicken GH-cDNA and amino acid sequences with related species.

Main Methods:

  • Synthesis of GH-cDNA from chicken pituitary poly A(+)-mRNA.
  • Cloning of chicken-cDNA library into E. coli.
  • DNA sequencing and comparative analysis of GH-cDNA and amino acid sequences.

Main Results:

  • Chicken GH-cDNA showed 97% similarity to a previously published chicken GH-cDNA sequence.

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  • Chicken GH-cDNA exhibited only 70% similarity to duck GH-cDNA.
  • Sequence dissimilarities were mainly in the 3'-non-coding region between chicken and duck GH-cDNAs.
  • Chicken and duck GH amino acid sequences differed by only three substitutions, with chicken GH sequences being identical.

    • Conclusions:

    • The chicken GH gene is highly conserved within the species.
    • Significant divergence exists in the non-coding regions of GH-cDNA between chicken and duck.
    • The amino acid sequence of GH is highly conserved between chicken and duck, suggesting functional similarity.