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Updated: Jun 20, 2026

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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
An ultrasensitive electrochemical "turn-on" label-free biosensor for Hg2+with AuNP-functionalized reporter DNA as a
Rong-Mei Kong1, Xiao-Bing Zhang, Liang-Liang Zhang
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Abstract:
A highly selective electrochemical biosensor for the ultrasensitive detection of Hg2+ in aqueous solution has been developed based on the strong and specific binding of Hg2+ by two DNA thymine bases (T-Hg2+-T) and the use of AuNP-functionalized reporter DNA to achieve signal amplification.
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
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