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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Published on: March 24, 2023

Virtual-core flow cytometry.

Jarred E Swalwell¹, Timothy W Petersen, Ger van den Engh

¹Department of Oceanography, University of Washington, Seattle, Washington 98105, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|September 16, 2009

Summary

This summary is machine-generated.

This study introduces a novel virtual-core flow cytometer (VCFC) that eliminates the need for sheath fluid, simplifying instrumentation. The VCFC accurately measures particles and cells, enabling applications in environmental monitoring and immunology.

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Area of Science:

Biotechnology
Analytical Chemistry
Instrumentation Science

Background:

Traditional flow cytometers rely on sheath fluid, complicating instrument design and limiting applications.
Sheath fluid requirement poses challenges for miniaturization and field deployment of flow cytometry systems.

Purpose of the Study:

To develop and validate a sheath-fluid-free flow cytometer using position-sensitive detectors.
To demonstrate the efficacy of the virtual-core flow cytometer (VCFC) for particle and cell analysis.

Main Methods:

Designed a flow cytometer utilizing three photodetectors to determine particle position within the sample stream.
Created a 'virtual core' using position-sensitive detectors to obviate the need for sheath fluid.
Validated the VCFC with test particles, immunolabeled thymocytes, and raw seawater samples.

Main Results:

The virtual-core flow cytometer (VCFC) successfully eliminated the requirement for sheath fluid.
Accurate measurements of particles and cells were achieved using the VCFC.
Demonstrated the VCFC's capability to analyze diverse samples, including biological cells and environmental water.

Conclusions:

The developed virtual-core flow cytometer (VCFC) offers a simplified and potentially more versatile alternative to traditional flow cytometers.
The VCFC's sheath-fluid-free design broadens the scope of flow cytometry applications, including environmental monitoring and basic immunology.
This technology paves the way for more accessible and portable flow cytometry instrumentation.