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Updated: Jun 20, 2026
10:44
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
Published on: May 5, 2023
Epigenetics--gene silencing.
1Nuclear Medicine Department, Jaslok Hospital and Research Centre, Mumbai.
The Journal of the Association of Physicians of India
|September 17, 2009
Summary
RNA interference (RNAi) enables gene silencing for cellular processes and research. Radio-labeled antisense oligonucleotides (RASONs) offer novel therapeutic strategies for cancers and neurodegenerative diseases.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Discovery of RNA interference (RNAi) by ds RNA in 1998 by Fire and Mello.
- RNAi mechanism for gene regulation via transcriptional (TGS) or post-transcriptional gene silencing (PTGS).
- RNAi as a tool for experimental gene function elucidation.
Purpose of the Study:
- Review the impact of RNAi on biomedical research.
- Explore novel medical and therapeutic applications of RNAi.
- Highlight radio-labeled antisense oligonucleotides (RASONs) for cancer and neurodegenerative disease treatment.
Main Methods:
- Discussion of gene silencing mechanisms.
- Review of therapeutic applications of RASONs.
- Analysis of antisense oligonucleotides (ASONs) for alternative splicing modulation.
Main Results:
- RNAi is crucial for cellular processes and gene function studies.
- RASONs show promise in diagnosing and treating cancers and neurodegenerative diseases.
- ASONs can correct aberrant splicing mutations in genetic disorders.
Conclusions:
- RNAi has significantly impacted biomedical research.
- RNAi-based therapeutics, particularly RASONs, hold great potential for future medicine.
- ASONs offer a viable strategy for correcting splicing defects in inherited diseases.
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