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"Zipper" molecular beacons: a generalized strategy to optimize the performance of activatable protease probes

Juan Chen1, Tracy W B Liu, Pui-Chi Lo

  • 1Department of Medical Biophysics, Ontario Cancer Institute, University of Toronto, Toronto, Ontario, Canada.

Bioconjugate Chemistry
|September 17, 2009
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...