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Related Concept Videos

Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Silica Gel Column Chromatography: Overview01:10

Silica Gel Column Chromatography: Overview

Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...

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Related Experiment Video

Updated: Jun 20, 2026

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

Microporous coordination polymers as selective sorbents for liquid chromatography.

Rashid Ahmad1, Antek G Wong-Foy, Adam J Matzger

  • 1Department of Chemistry and the Macromolecular Science and Engineering Program, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109-1055, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|September 17, 2009
PubMed
Summary

Microporous coordination polymers (MCPs) show promise as stationary phases for liquid chromatography. These materials effectively separate organic compounds using molecular sieving and adsorption, demonstrating their potential in advanced separation science.

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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites
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A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Published on: March 1, 2018

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Separation Science

Background:

  • Microporous coordination polymers (MCPs) are advanced materials with tunable porous structures.
  • Traditional stationary phases in liquid chromatography face limitations in separating complex mixtures.
  • Exploring novel materials like MCPs is crucial for advancing chromatographic techniques.

Purpose of the Study:

  • To investigate the efficacy of specific MCPs, namely HKUST-1 and MOF-5, as stationary phases in liquid chromatography.
  • To assess the separation capabilities of these MCPs for a range of organic compounds.
  • To understand the separation mechanisms, including molecular sieving and adsorption, employed by MCPs.

Main Methods:

  • Synthesis and characterization of copper-carboxylate (HKUST-1) and zinc-carboxylate (MOF-5) based MCPs.
  • Utilizing these MCPs as stationary phases in liquid chromatographic systems.
  • Performing separations of various aromatic organic compounds with differing sizes and shapes.

Main Results:

  • Excellent separation of organic compounds including benzene, ethylbenzene, styrene, naphthalene, anthracene, phenanthrene, pyrene, 1,3,5-triphenylbenzene, and 1,3,5-tris(4-bromophenyl)benzene was achieved.
  • Separation performance was attributed to a combination of molecular sieving (size and shape selectivity) and adsorption effects.
  • HKUST-1 and MOF-5 demonstrated significant potential for selective chromatographic separations.

Conclusions:

  • Microporous coordination polymers (MCPs) are effective stationary phases for liquid chromatography.
  • The studied MCPs offer shape and size selective separation capabilities for organic molecules.
  • MCPs present a promising platform for developing next-generation chromatographic separation materials.