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Updated: Jun 20, 2026
Establishment of Patient-Derived Xenograft Mouse Model with Human Osteosarcoma Tissues
Published on: March 22, 2024
Development of a new canine osteosarcoma cell line
B Séguin1, T Zwerdling, J L McCallan
1Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA, USA. bernard.seguin@oregonstate.edu
Abstract:
Establishing a canine osteosarcoma (OSA) cell line can be useful to develop in vivo and in vitro models of OSA. The goal of this study was to develop, characterize and authenticate a new canine OSA cell line and a clone. A cell line and a clone were developed with standard cell culture techniques from a naturally occurring OSA in a dog. The clonal cell line induced a tumour after injection in RAG 1-deficient mouse. Histology was consistent with OSA. The original tumour from the dog and the tumour induced in the mouse were both reactive with vimentin and osteonectin (ON). The parent cell line and clonal cell line were reactive with ON, osteocalcin and alkaline phosphatase. Loss of heterozygosity was found in the same three microsatellite markers in the parent and clonal cell lines, and the tumour tissue grown in the mouse.
