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Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:

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Related Experiment Video

Updated: Jun 20, 2026

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
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Published on: June 5, 2014

PCR for M. tuberculosis in tissue samples.

Kiran Chawla1, Soham Gupta, Chiranjay Mukhopadhyay

  • 1Department of Microbiology and Pathology, Kasturba Medical College, Manipal, Karnataka, India. arunkiranchawla@yahoo.com

Journal of Infection in Developing Countries
|September 17, 2009
PubMed
Summary
This summary is machine-generated.

A new PCR test offers rapid and sensitive detection of Mycobacterium tuberculosis in tissue samples. This molecular method aids in early tuberculosis diagnosis, improving treatment initiation and patient outcomes.

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Last Updated: Jun 20, 2026

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10:41

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Published on: April 30, 2020

Area of Science:

  • Microbiology
  • Pathology
  • Molecular Diagnostics

Background:

  • Granulomas in histopathology are not specific for tuberculosis diagnosis.
  • Histopathological reporting for tissue samples takes 1-2 weeks.
  • There is a need for rapid Mycobacterium tuberculosis detection in paucibacillary samples.

Purpose of the Study:

  • To evaluate a PCR-assay for IS6110 for Mycobacterium tuberculosis detection in tissue samples.
  • To compare PCR assay performance against histopathology as the gold standard.

Main Methods:

  • A PCR-assay targeting the IS6110 gene was used.
  • 104 diverse tissue samples were analyzed.
  • Results were compared with histopathology findings.

Main Results:

  • PCR demonstrated 74.1% sensitivity and 96.1% specificity.
  • False positive rate was 2.88% (3/104) and false negative rate was 6.73% (7/104).
  • Positive agreement between PCR and histopathology was 0.737, indicating substantial agreement.

Conclusions:

  • PCR provides a rapid and sensitive method for early tuberculosis diagnosis in tissue samples.
  • Timely diagnosis facilitates prompt anti-tubercular treatment initiation.
  • Early intervention can prevent irreversible pathological changes.