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Updated: Jun 20, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
Published on: January 24, 2016
Interferon regulatory factor-1 is a key transcription factor in murine beta cells under immune attack
C Gysemans1, H Callewaert1, F Moore2
1LEGENDO, Campus Gasthuisberg O&N1, Herestraat 49, bus 902, 3000, Leuven, Belgium.
Aims/Hypothesis:
IFN-gamma, together with other inflammatory cytokines such as IL-1beta and TNF-alpha, contributes to beta cell death in type 1 diabetes. We analysed the role of the transcription factor interferon regulatory factor (IRF)-1, a downstream target of IFN-gamma/signal transducer and activator of transcription (STAT)-1, in immune-mediated beta cell destruction.
Methods:
Islets from mice lacking Irf-1 (Irf-1 (-/-)) and control C57BL/6 mice were transplanted in overtly diabetic NOD mice. Viability and functionality of islets were evaluated in vitro. Chemokine expression by Irf-1 (-/-) islets and INS-1E cells transfected with Irf-1 short interfering RNA (siRNA) was measured by real-time PCR as well as in functional assays in vitro.
Results:
IRF-1 deletion in islets was associated with higher prevalence of primary non-function (63% vs 25%, p
Conclusions/Interpretation:
These data indicate a key regulatory role for IRF-1 in insulin and chemokine secretion by pancreatic islets under inflammatory attack.
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